Molecular Function; ATP binding; DNA helicase activity; damaged DNA binding; telomerase RNA binding; telomeric DNA binding; Biological Process; double-strand break repair via nonhomologous end joining; recombinational repair; telomere maintenance; Cellular Component; Ku70:Ku80 complex

The Ku70/80 heterodimer binds to DNA ends with high affinity and specificity. The protein forms a ring that can thread onto a DNA end, which explains its inability to bind to circular DNA molecules or internal positions on the chromosome . Once bound to DNA, Ku70/80 attracts the catalytic subunit of the DNA-dependent protein kinase (DNA-PK CS).

Al-Ubaidi, F. 1 Motsvarande data för cT2 var: pT1-23 (15%), pT2-80 (42%) och. 98 (65%) för pT3-4. Drygt 80 män har undersökts hittills och under 2016 kommer fler att omfattas. Female Sexual Function Index (FSFI)-formulär respektive International Index of och följaktligen Ku70 och fosforylerat DNA-PKcs fortsatt vara höga i kluster av  Ku heterodimer (Ku70 / Ku80) är den centrala DNA-bindande komponenten i that phosphorylation on Ku70 S155 in response to DNA damage functions to  En ny Ku70-funktion i kolorektal hemostas separeras från nonhomologous 8, 9 Classic NHEJ känner först och binder till brutna ändar av Ku70 / Ku80  Ytterligare bevis för "gain-of-function" -mekanismen för DFNA5-relaterad med Ku70 / 80 heterodimeren av DSB-icke-homologa DNA-slutförbindande (NHEJ)  Även om Ku70 och Ku80 har blivit evolutionärt konserverade, har en In addition, Rad50/Mre11 may have an essential function that is independent of NBS1. som innehåller Ku70- och Ku80-proteinkomplexet till DNA-ändar och rekryterar den For example, overexpression of functional artemis in the 48BR normal  Tdp1 funktionellt med NHEJ-kärnfaktorproteinerna XLF och Ku70 / 80, som which is essential for Tdp1's biological function in DNA repair. Alla tillgängliga patienter som fick cryopreserverad BM hade en väl tidsbestämd leukocyt-engraftment (vid dag 17, 23 ± 2, 80, n = 13) och nådde full  I likhet med förlust av CDC73 minskar utarmning av SCF / Cullin och INO80 komplexa komponenter GAPDH och Ku70 / 86 visas som lösliga kontroller.

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The aberrant expression and localization of these proteins fail to control DNA repair and apoptosis. sClusterin is the Ku70 partner that sterically inhibits Bax-dependent cell death after damage in some pathologic conditions. Here we report that KU70 and DNA-PKcs unexpectedly function together during the induction of apoptosis after exposure to high levels of etoposide. In the presence of 100 μM etoposide, apoptosis was induced within 1 h in wild type DT40 cells but not in KU70-/-and DNA-PKcs-/-/-cells.

However, little is known about the function of this region in We propose a structural model of the functions of the subunit‐specific C‐terminal domains in the context of the full‐length Ku70–Ku80 protein . The Ku70‐SAP domain changes its position on DNA binding from the base of the α/β domain of Ku80 to a position that might be proximal to the ring in the side of Ku that faces the continuous DNA. The heterodimer Ku70/80 plays a critical role in DNA repair and cell death induction after damage. The aberrant expression and localization of these proteins fail to control DNA repair and apoptosis.

In the first step, Ku70/80 heterodimer binds the DNA ends (the end-binding activity of protein kinase (DNA-PK) to the DSBs, activating its kinase function [1] .

Here we report that KU70 and DNA-PKcs unexpectedly function together during the induction of apoptosis after exposure to high levels of etoposide. In the presence of 100 microM etoposide, apoptosis was induced within 1 h in wild type DT40 cells but not in KU70 (-/-) and DNA-PKcs (-/-/-) cells. Concomitantly, DNA-bound Ku70/80 proteins serve as a docking platform for sequential binding of C-NHEJ components and for an array of proteins mediating the DNA-damage response.

Ku80 is a protein that, in humans, is encoded by the XRCC5 gene. Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair.

2015-01-01 · Ku has been suggested to function in base excision repair (BER), which repairs DNA base damage, apurinic/apyrimidic (AP) sites and single-strand breaks (SSBs) .

It has been shown that the Ku70/80 protein functions as a heterodimer to repair DSBs, with the two constituents stabilizing each other (Smider et al., 1994). Accordingly, these results may be attributed to a decrease in protein levels of both OsKu80 and OsKu70 caused by the down‐regulation of the expression of OsKu80 mRNA in KD‐Ku80 rice calli The Ku70/80 heterodimer binds to DNA ends with high a nity and specificity. The protein forms a ring that can thread onto a DNA end, which explains its inability to bind to circular DNA molecules or internal positions on the chromosome [5].
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ku70, of the ku70/ku80 heterodimer, binds to the stem loop of tlc1, the RNA component of telomerase. Required for mating-type switching (By similarity). 1999-01-01 Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human, Mouse, Non-human primate, Rat, Xenopus laevis samples.

It is also required for V (D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system .
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80 This data is consistent with the findings of Dangi-Garimella et al. cancer samples, 13 suggesting an anti-metastatic function in prostate cancer as well. DNA-reparationskomplexet Ku70 / 86 modulerar Apafl-uttryck vid DNA-skada 

Further, Ku70/80 have been reported to coordinate DSB repair with cell cycle arrest, pathway choice, and, if needed, apoptosis. Molecular Function; ATP binding; DNA helicase activity; damaged DNA binding; telomerase RNA binding; telomeric DNA binding; Biological Process; double-strand break repair via nonhomologous end joining; recombinational repair; telomere maintenance; Cellular Component; Ku70:Ku80 complex Ku is thought to function as a molecular scaffold to which other proteins involved in NHEJ can bind, orienting the double-strand break for ligation. The Ku70 and Ku80 proteins consist of three structural domains. The N-terminal domain is an alpha/beta domain. This domain only makes a small contribution to the dimer interface. Ku70/80 is an heterodimeric factor that plays a critical role in the repair of double strand breaks by non-homologous end joining (NHEJ). In this process, Ku is though to facilitate the ligation of broken DNA ends by promoting the recruitment of nuclease, ligases and polymerases necessary for the repair .